文章摘要
徐礼生.谷氨酰转肽酶细胞合成L-谷氨酰正丙胺的研究[J].精细化工,2018,35(6):
谷氨酰转肽酶细胞合成L-谷氨酰正丙胺的研究
Synthesis of Propyl-L-Glutamine by glutamyltranspeptidase cell
投稿时间:2017-07-13  修订日期:2017-10-11
DOI:
中文关键词: 谷氨酰胺转肽酶  L-谷氨酰正丙胺  L-谷氨酰肼  合成
英文关键词: glutamyltranspeptidase  propyl-L-glutamine  L-glutamlhydrine  synthesis
基金项目:
作者单位E-mail
徐礼生 宿州学院 xulisheng111@163.com 
摘要点击次数: 41
全文下载次数: 87
中文摘要:
      摘要:利用谷氨酰转肽酶细胞合成L-谷氨酰正丙胺,采用L-谷氨酰肼和正丙胺作为底物,考察底物浓度、温度、pH、催化剂用量和底物物质的量对合成L-谷氨酰正丙胺的影响。研究结果表明:温度为37 ℃、pH为10、L-谷氨酰肼浓度为0.4 mol/L,正丙胺浓度为3 mol/L,产物L-谷氨酰正丙胺的产率为97.9%,L-谷氨酰正丙胺收率为85.3%,L-谷氨酰正丙胺纯度为99.8%。谷氨酰转肽酶细胞能有效合成L-谷氨酰正丙胺。
英文摘要:
      Abstract: Synthesis of propyl-L-glutamine by glutamyltranspeptidase cells was investigated. With L-glutamlhydrine and propylamine as substrate, the factors such as substrate concentration, pH and temperature were investigated. The results indicated that when the concentration of L-glutamlhydrine was 0.4 mol/L, the concentration of propylamine was 3 mol/L, reaction temperature was 37ºC, and pH was 10, the yield of obtained propyl-L-glutamine reached 97.9%.The yield of propyl-L-glutamine was 85.3%. The purity of propyl-L-glutamine was 99.8%. Synthesis of propyl-L-glutamine by glutamyltranspeptidase cells was effective.
查看全文   查看/发表评论  下载PDF阅读器
关闭