文章摘要
匡红波,王忠龙,张燕,芮坚,王芸芸,徐徐,谷文,王石发.新型喹啉类Zn2+探针的合成及细胞成像研究[J].精细化工,2018,35(6):
新型喹啉类Zn2+探针的合成及细胞成像研究
Novel Quinoline-Based Fluorescent Probes for Detecting Zn2+ in Living Cells
投稿时间:2017-07-06  修订日期:2017-10-10
DOI:
中文关键词: 8-羟基喹啉  希夫碱  锌离子  荧光探针  细胞成像
英文关键词: 8-hydroxyquinoline  Schiff-base  zinc ion  fluorescence probe  cell imaging
基金项目:江苏省高校自然科学研究重大项目(No.14KJ220001)资助项目;国家自然科学基金(No.31470592);江苏省生物质能源与材料重点实验室开放基金项目(No.JSBEM2014010)
作者单位E-mail
匡红波 南京林业大学 982882930@qq.com 
王忠龙 南京林业大学  
张燕 南京林业大学  
芮坚 南京林业大学  
王芸芸 南京林业大学  
徐徐 南京林业大学  
谷文 南京林业大学  
王石发 南京林业大学 wangshifa65@163.com 
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中文摘要:
      以2-甲酰基-8-羟基喹啉为原料,与苯肼进行缩合反应合成了荧光分子探针HSZn,采用FTIR、NMR、HRMS对其结构进行表征。紫外可见光谱和荧光光谱分析研究表明,该探针在水相中对Zn2+具有较好的荧光增强效应,而其他金属离子对其干扰较小;HSZn的荧光强度与Zn2+浓度(1.0×10-5~1.0×10-4 mol/L)具有良好的线性关系,检测限低至4.0×10-8 mol/L;Job’s曲线和LC-MS分析表明,Zn2+与HSZn的络合比为1:2,且探针在pH=5~13内对Zn2+都有较灵敏的荧光响应。细胞成像实验表明,该探针可在Hela活细胞中实现对Zn2+的荧光成像。
英文摘要:
      A Schiff-based fluorescent molecular probe (HSZn) was synthesized by condensation of quinoline with phenylhydrazine and characterized by FTIR, NMR and HRMS. The UV-Vis spectroscopy and fluorescence spectroscopy studies demonstrated that the probe showed a significant fluorescence enhancement effect toward Zn2+ in aqueous medium while suffering from a slight interference at the presence of other metal ions. A good linear relationship between the Zn2+ concentrations (1.0×10-5~1.0×10-4 mol/L) and the fluorescence intensity was obtained for determining the content of Zn2+ with a detection limit of 4.0×10-8 mol/L. A 1:2 binding ratio of Zn2+/HSZn for the complex was obtained by Job’s plot and LC-MS, HSZn also exhibited a sensitive fluorescence response toward Zn2+ in the wide pH range (pH=5~13). Cell imaging studies indicated that the probe was capable of detecting endogenous Zn2+ in live Hela cells.
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